Cortex Technology offers a complete range of accessories divided into the following categories: closed contact probes, open spray-tips, extensions & adapters, cones and storage containers.
Mechanism of cell injury
The mechanism of cell injury from freezing is both chemical and mechanical, and a main effect is directly related to the formation of ice crystals within the cells. Rapid freezing does not allow sufficient time for intracellular water to leave the cell and, consequently, it leads to intracellular ice crystallisation and mechanical rupture of the cell membrane. During slow thawing, recrystallisation takes place – i.e. small ice crystals aggregate to form larger crystals – leading to further mechanical damage of the cell membranes.
The CryoPro Spray-tips are available with four apertures: A (1.00 mm) provides a heavy spray for large lesions and superficial desquamation. B & C (0.75, 0.55 mm) with a medium output for general application and D (0.45 mm) with a fine spray for smaller lesions.
Select a spray aperture appropriate for the lesion to be treated. In depth freezing is best obtained, when the spray aperture is close to the lesion (5-10 mm distance). Superficial desquamation is obtained using the soft spray tip at approx. 5 cm distance.
Open Spray technique
When using the open spray tip select a spray aperture appropriate for the lesion to be treated. To obtain in-depth freezing it is essential to place the spray aperture close to the lesion (5 – 10 mm distance), as the liquid content of the spray is higher close to the aperture.
When possible, raised warts should be approached tangentially from at least two sides. This allows the ice ball to move down through the wart while sparing the surrounding tissue. Spraying directly at the lesion (perpendicular to the surface) will cause more lateral spread with less penetration. To avoid this, apply the spray intermittently or use a smaller spray aperture.
Superficial desquamation is obtained using the larger apertures (“A” or “B”) at approx. 5 cm distance (higher vapor content) and slowly “painting” the surface with vaporised nitrogen.
Cleaning a clogged spray tip
Do never attempt to clean the aperture with a hard and sharp pointed object (e.g. a needle), as this may cause irreversible damage to the aperture. Instead, detach the aperture and blow it clean with pressurized air in the reverse direction of the normal nitrogen flow. Alternatively, blow it clean with liquid nitrogen in the reverse direction. In order to perform this quick and easy operation, a special cleaning adapter is available.
Small diameter spray apertures (C & D sizes) are more sensitive to impurities in the liquid nitrogen supply than large diameters (A & B sizes). In the event of a very uneven spray (spitting) or no spray at all, this might be an indication of partial or complete clogging of the aperture.
Contact probes are split into three categories: small sized, medium sized and large probes. All probes are gold plated to facilitate cleaning.
Small Sized probes are ideal for tiny to small lesions e.g. small flat warts, lesions near the eye or on the eyelid.
Medium Sized probes are ideal for small to medium size lesions e.g. flat warts, small benign tumors, lesions near the eye.
Large Sized probes are likewise ideal for for medium size to large lesions e.g. flat warts, and benign tumors.
Closed contact probes provide in-depth freezing with minimal lateral spreading of the freeze. Select a probe fitting the size of the lesion. Wet the skin with a drop of water prior to probe application to improve efficacy.
Closed contact probe technique
Closed contact probes provide in-depth freezing with minimal lateral spread. Select a probe size fitting the size of the lesion. Make the lesion wet with a drop of water or contact gel prior to freezing to facilitate the thermal transfer between skin and probe tip. Apply the contact probe when still warm applying light pressure and pull the trigger handle. Allow approx. 5 sec. extra freezing time to cool down the contact probe and keep freezing until a 1 mm halo is seen around the probe tip. Then stop the freezing and allow a few seconds for the probe tip to come loose. This “quick freeze slow thaw” procedure using the contact probe technique ensures a very effective treatment with high penetration and accuracy.
Extensions & adapters
Cortex Technology also provides a range of extensions such as straight spray ext., bent spray ext., soft peel desquamation tip, a luer lock adapter for needles, spray tip cleaning adapter and open flow restrictor.
Cortex spray accessories
Spray cones in combination with an open spray aperture may be used to confine the lateral spread of the freeze. Small diameter hard, clear cones are available for small to medium size lesions. For larger lesions rubber cones are available, which may be shaped to follow the margins of the lesion more accurately